Thursday, February 4, 2010

Paper Chromatography Lab















Paper Chromatography Lab

In this lab the use of chromatography paper and different solvents to test the retention time of the solvents.

The retention time of each solvents
Tested to see which of them had a better retention time to disperse the independent pigments
The solvents were tested to see which had a better retention time and how far to disperse the pigments up the chromatography paper
Test which color had which pigments mixed in the mixture or if it was a compound

Hypothesis
our hypothesis is that the h2o will be the best of the different fluids to be used in this experiment.

Materials
-Chromatography paper strips
-Water
-Methanol
-Isopropanol
-Hexane
-Different colors of overhead pens one of which is black
-Well plate
-Make sure to wear safety equipment including aprons and safety goggles at all times

We made three black dots on 4 chromatography strips in a line along the narrow part on one end of the strip. Then we filled a well plate for each substance half the way full. We put a crease in the chromatography in the end with the dots and put one in each well and recorded the retention time each substance took to separate the color and how far the the pigments were separated.
Then we took the best solvent and put three dots on each chromatography with different colors. And recorded the results.

Results
The water worked the best. Methanol was second best and then Hexane , and Isopropyl did work at all. Since water has hydrogen in it, it has the strongest bond making it capable of pulling the pigments farther.

8 comments:

  1. nice start but still plenty of work to do... clear up your formatting/grammar, and get images from your notebooks in there

    ReplyDelete
  2. Picture is too dark.. Need to capitalize beginning of sentences and use proper punctuation.

    ReplyDelete
  3. List of materials was very well planned out...your conclusions needs to be a bit better..overall very nice!

    ReplyDelete
  4. This lab seems kinda short but looks good. Stuhler, thanks for the sub. I hate you Tyson. Hi Seth. HUTCH

    ReplyDelete
  5. Ooh like the background. You should throw some of the chemical formulas in there and make more separation in the different subjects, maybe put the subject matter in bold.Otherwise good job!

    ReplyDelete
  6. I like your colorful strips of paper, seemed like that lab went well. Try adding the rest of your information, it helps to look off the lab sheet itself for some ideas and factual information. Great Start. <>< God Bless

    ReplyDelete
  7. overall great job! maybe just add some definition between your paragraphs and the labels to make things easier to read

    ReplyDelete